Search results for "Shikimate dehydrogenase"
showing 3 items of 3 documents
Determination of molecular mass, Stokes' radius, frictional coefficient and isomer-type of non-denatured proteins by time-dependent pore gradient gel…
1988
Molecular mass, Stoke's radius, frictional coefficient and isomer-type of non-denatured proteins can be obtained by time-dependent gradient gel electrophoresis by evaluating the resulting data using a two-step mathematical procedure. Provided a histochemical staining procedure is available to locate the position of an enzyme in the gel, crude cell extracts can be used for estimating their molecular size properties. The computation of molecular properties of non-denatured proteins is demonstrated for isozymes of aspartate aminotransferase (EC 2.6.1.1), peroxidase (EC 1.11.1.42) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) from current-year needles of spruce. The resulting data as well…
Intracellular compartmentation and regulation of two shikimate dehydrogenase isoenzymes in Pisum sativum
1974
Summary Pea seeds as well as sprouts and roots contain two isoenzymes of shikimate dehydrogenase. Both isoenzymes can be separated by Polyacrylamide gel electrophoresis as well as through ammonium sulfate fractionation. The molecular weight of both isoenzymes are the same although the net electric charge is different. The Km value for isoenzyme 1 is 3,5 × 10 −4 and the Km value for isoenzyme 2 is 1,67 × 10 −4 M. 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, gallic acid, anthranilic acid and p-methoxycinnamic acid inhibited both isoenzymes competitively. Anthranlic acid showed the largest affinity to both isoenzymes. M-methoxycinnamic acid and m-nitrocinnamic acid inhibited both isoe…
Preparation of extracts from mature spruce needles for enzymatic analyses
1987
It was possible to extract simultaneously several active enzymes involved in the carbohydrate or the amino acid metabolism from spruce needles [Picea abies (L.) Karst.] when a) a 100 mM Na-Pi buffer of pH 7.5 containing 5% PVPP and 0.5% Triton X-100 was used and when b) the resulting crude extracts were freed from lowmolecular-weight compounds by gel-chromatography using the separation medium Fractogel TSK HW-40. Besides Triton X-100, Triton X-305, Myrij-52 and Brij-35 were tested, but 0.5% Triton X-100 brought about the most active enzyme extracts. In crude extracts prepared from spruce needles during the early summer a high increase in absorbance at 334 nm was observed when the co-substra…